PrimePCR™ SYBR® Green Assay: Nkx2-5, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.


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PrimePCR™ PreAmp for SYBR® Green Assay: Nkx2-5, Mouse
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: Nkx2-5, Mouse
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qMmuCID0020858
Assay Design:		Intron-spanning
Chromosome Location:		17:26839582-26841134question
Amplicon Length:		146
Splice Variants Targeted:		ENSMUST00000044783 ENSMUST00000115463 ENSMUST00000142344 ENSMUST00000115457 ENSMUST00000115461 ENSMUST00000115460 ENSMUST00000073840 ENSMUST00000015723 ENSMUST00000063136 ENSMUST00000114099 ENSMUST00000033994 ENSMUST00000053251 ENSMUST00000033995

Gene Information

Description Not Available

Gene Symbol:		Nkx2-5
Gene Name:		NK2 transcription factor related, locus 5 (Drosophila)
Aliases:		Csx, Nkx-2.5, Nkx2.5, tinman
RefSeq:		NC_000083.6 NT_039649.8
Ensembl:		ENSMUSG00000015579
Entrez:		18091
Chromosome Mapping:		17 A3.3\|17 13.0 cM

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Mouse Reference Total RNA

Summary Data:

R²	0.998800
y-intercept	36.870000
Efficiency	95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.