PrimePCR™ SYBR® Green Assay: CPT1B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.


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PrimePCR™ PreAmp for SYBR® Green Assay: CPT1B, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: CPT1B, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0003228
Assay Design:		Exonic
Chromosome Location:		22:51007290-51007429question
Amplicon Length:		110
Splice Variants Targeted:		ENST00000405237 ENST00000312108 ENST00000360719 ENST00000457250 ENST00000440709

Gene Information

The protein encoded by this gene a member of the carnitine/choline acetyltransferase family is the rate-controlling enzyme of the long-chain fatty acid beta-oxidation pathway in muscle mitochondria. This enzyme is required for the net transport of long-chain fatty acyl-CoAs from the cytoplasm into the mitochondria. Multiple transcript variants encoding different isoforms have been found for this gene and read-through transcripts are expressed from the upstream locus that include exons from this gene. [provided by RefSeq Jun 2009]

Gene Symbol:		CPT1B
Gene Name:		carnitine palmitoyltransferase 1B (muscle)
Aliases:		CPT1-M, CPT1M, CPTI, CPTI-M, FLJ55729, FLJ58750, KIAA1670, M-CPT1, MCCPT1, MCPT1
RefSeq:		NC_000022.10 NT_011526.7 NG_012643.1
Ensembl:		ENSG00000205560
Entrez:		1375
Chromosome Mapping:		22q13.33

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.997100
y-intercept	34.230000
Efficiency	101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.