PrimePCR™ SYBR® Green Assay: ACSL6, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0040371


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PrimePCR™ PreAmp for SYBR® Green Assay: ACSL6, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: ACSL6, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0037648
Assay Design:		exonic
Chromosome Location:		5:131326579-131329732question
Amplicon Length:		60
Splice Variants Targeted:		ENST00000379264 ENST00000379255 ENST00000296869 ENST00000379246 ENST00000379244 ENST00000379240 ENST00000431707 ENST00000434099 ENST00000430403 ENST00000419502 ENST00000416557 ENST00000414078 ENST00000441995 ENST00000379249 ENST00000379272 ENST00000357096 ENST00000543479 ENST00000544770

Gene Information

The protein encoded by this gene catalyzes the formation of acyl-CoA from fatty acids ATP and CoA using magnesium as a cofactor. The encoded protein plays a major role in fatty acid metabolism in the brain. Translocations with the ETV6 gene are causes of myelodysplastic syndrome with basophilia acute myelogenous leukemia with eosinophilia and acute eosinophilic leukemia. Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq Apr 2011]

Gene Symbol:		ACSL6
Gene Name:		acyl-CoA synthetase long-chain family member 6
Aliases:		ACS2, FACL6, FLJ16173, KIAA0837, LACS 6, LACS2, LACS5
RefSeq:		NC_000005.9 NT_034772.6
Ensembl:		ENSG00000164398
Entrez:		23305
Chromosome Mapping:		5q31

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.998900
y-intercept	35.610000
Efficiency	99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.