PrimePCR™ SYBR® Green Assay: FABP4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Recommended - best efficiency; Same primer pair as used in probe assay qHsaCEP0058579


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PrimePCR™ PreAmp for SYBR® Green Assay: FABP4, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: FABP4, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0057474
Assay Design:		exonic
Chromosome Location:		8:82391012-82391143question
Amplicon Length:		102
Splice Variants Targeted:		ENST00000256104

Gene Information

FABP4 encodes the fatty acid binding protein found in adipocytes. Fatty acid binding proteins are a family of small highly conserved cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. It is thought that FABPs roles include fatty acid uptake transport and metabolism. [provided by RefSeq Jul 2008]

Gene Symbol:		FABP4
Gene Name:		fatty acid binding protein 4, adipocyte
Aliases:		A-FABP, AFABP, ALBP, aP2
RefSeq:		NC_000008.10 NT_008183.19
Ensembl:		ENSG00000170323
Entrez:		2167
Chromosome Mapping:		8q21

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.997300
y-intercept	35.690000
Efficiency	100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.