PrimePCR™ SYBR® Green Assay: STAT2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028748

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: STAT2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: STAT2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0013150
Assay Design:   Intron-spanning
Chromosome Location:   12:56743941-56744915question
Amplicon Length:   119
Splice Variants Targeted:   ENST00000314128 ENST00000418572 ENST00000557235

Gene Information

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors STAT family members are phosphorylated by the receptor associated kinases and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. In response to interferon (IFN) this protein forms a complex with STAT1 and IFN regulatory factor family protein p48 (ISGF3G) in which this protein acts as a transactivator but lacks the ability to bind DNA directly. Transcription adaptor P300/CBP (EP300/CREBBP) has been shown to interact specifically with this protein which is thought to be involved in the process of blocking IFN-alpha response by adenovirus. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Mar 2010]

Gene Symbol:   STAT2
Gene Name:   signal transducer and activator of transcription 2, 113kDa
Aliases:   ISGF-3, MGC59816, P113, STAT113
RefSeq:   NC_000012.11 NT_029419.12
Ensembl:   ENSG00000170581
Entrez:   6773
Chromosome Mapping:   12q13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 34.850000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download