PrimePCR™ SYBR® Green Assay: IL6, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: IL6, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: IL6, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0020314
Assay Design:   Intron-spanning
Chromosome Location:   7:22768389-22769247question
Amplicon Length:   122
Splice Variants Targeted:   ENST00000404625 ENST00000426291 ENST00000401651 ENST00000407492 ENST00000401630 ENST00000406575 ENST00000258743 ENST00000420258

Gene Information

This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. [provided by RefSeq Jun 2011]

Gene Symbol:   IL6
Gene Name:   interleukin 6 (interferon, beta 2)
Aliases:   BSF2, HGF, HSF, IFNB2, IL-6
RefSeq:   NC_000007.13 NG_011640.1 NT_007819.17
Ensembl:   ENSG00000136244
Entrez:   3569
Chromosome Mapping:   7p21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 34.060000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download