PrimePCR™ SYBR® Green Assay: PLCB1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0033154

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PLCB1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PLCB1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0021711
Assay Design:   Intron-spanning
Chromosome Location:   20:8665670-8678393question
Amplicon Length:   147
Splice Variants Targeted:   ENST00000378641 ENST00000338037 ENST00000378637 ENST00000441163 ENST00000535719 ENST00000338061

Gene Information

The protein encoded by this gene catalyzes the formation of inositol 145-trisphosphate and diacylglycerol from phosphatidylinositol 45-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of many extracellular signals. This gene is activated by two G-protein alpha subunits alpha-q and alpha-11. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   PLCB1
Gene Name:   phospholipase C, beta 1 (phosphoinositide-specific)
Aliases:   EIEE12, FLJ45792, PI-PLC, PLC-154, PLC-I, PLC154, PLCB1A, PLCB1B
RefSeq:   NC_000020.10 NT_011387.8 NG_028168.1
Ensembl:   ENSG00000182621
Entrez:   23236
Chromosome Mapping:   20p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 34.700000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download