PrimePCR™ SYBR® Green Assay: Pde4d, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qMmuCEP0054936


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PrimePCR™ PreAmp for SYBR® Green Assay: Pde4d, Mouse
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: Pde4d, Mouse
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qMmuCED0046510
Assay Design:		exonic
Chromosome Location:		13:109937990-109938108question
Amplicon Length:		89
Splice Variants Targeted:		ENSMUST00000122041 ENSMUST00000120671 ENSMUST00000153234 ENSMUST00000119507 ENSMUST00000079975 ENSMUST00000135275 ENSMUST00000074103 ENSMUST00000120664 ENSMUST00000117879 ENSMUST00000117420 ENSMUST00000155459 ENSMUST00000177907

Gene Information

Description Not Available

Gene Symbol:		Pde4d
Gene Name:		Phosphodiesterase 4D, cAMP specific
Aliases:		9630011N22Rik, Dpde3
RefSeq:		NC_000079.6 NT_039590.8
Ensembl:		ENSMUSG00000021699
Entrez:		238871
Chromosome Mapping:		13 D2.1-D2.2\|13 52.0 cM

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Mouse Reference Total RNA

Summary Data:

R²	0.999100
y-intercept	34.950000
Efficiency	103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.