PrimePCR™ SYBR® Green Assay: HEY2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0051873


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PrimePCR™ PreAmp for SYBR® Green Assay: HEY2, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: HEY2, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0044611
Assay Design:		exonic
Chromosome Location:		6:126081228-126081377question
Amplicon Length:		120
Splice Variants Targeted:		ENST00000368365 ENST00000368364

Gene Information

This gene encodes a member of the hairy and enhancer of split-related (HESR) family of basic helix-loop-helix (bHLH)-type transcription factors. The encoded protein forms homo- or hetero-dimers that localize to the nucleus and interact with a histone deacetylase complex to repress transcription. Expression of this gene is induced by the Notch signal transduction pathway. Two similar and redundant genes in mouse are required for embryonic cardiovascular development and are also implicated in neurogenesis and somitogenesis. Alternatively spliced transcript variants have been found but their biological validity has not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:		HEY2
Gene Name:		hairy/enhancer-of-split related with YRPW motif 2
Aliases:		CHF1, GRIDLOCK, GRL, HERP1, HESR2, HRT2, MGC10720, bHLHb32
RefSeq:		NC_000006.11 NT_025741.15
Ensembl:		ENSG00000135547
Entrez:		23493
Chromosome Mapping:		6q21

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999900
y-intercept	35.860000
Efficiency	96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.