PrimePCR™ SYBR® Green Assay: DAB2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0052852

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: DAB2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: DAB2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045591
Assay Design:   exonic
Chromosome Location:   5:39394394-39394483question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000339788 ENST00000320816 ENST00000509337 ENST00000511792 ENST00000503513 ENST00000515700 ENST00000545653

Gene Information

This gene encodes a mitogen-responsive phosphoprotein. It is expressed in normal ovarian epithelial cells but is down-regulated or absent from ovarian carcinoma cell lines suggesting its role as a tumor suppressor. This protein binds to the SH3 domains of GRB2 an adaptor protein that couples tyrosine kinase receptors to SOS (a guanine nucleotide exchange factor for Ras) via its C-terminal proline-rich sequences and may thus modulate growth factor/Ras pathways by competing with SOS for binding to GRB2. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Oct 2011]

Gene Symbol:   DAB2
Gene Name:   disabled homolog 2, mitogen-responsive phosphoprotein (Drosophila)
Aliases:   DOC-2, DOC2, FLJ26626
RefSeq:   NC_000005.9 NT_006576.16
Ensembl:   ENSG00000153071
Entrez:   1601
Chromosome Mapping:   5p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 35.850000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download