PrimePCR™ SYBR® Green Assay: CYP11B1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0053179

List Price:    $174.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: CYP11B1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CYP11B1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045918
Assay Design:   exonic
Chromosome Location:   8:143955340-143955513question
Amplicon Length:   144
Splice Variants Targeted:   ENST00000579123 ENST00000524765 ENST00000496961 ENST00000310611 ENST00000420578 ENST00000430195 ENST00000359827 ENST00000321063 ENST00000391744 ENST00000251390 ENST00000391745 ENST00000367278 ENST00000255432 ENST00000439764 ENST00000368457 ENST00000368456 ENST00000372271 ENST00000372269 ENST00000519285 ENST00000292427 ENST00000517471 ENST00000377675 ENST00000576482 ENST00000575459 ENST00000574138 ENST00000574010 ENST00000570542 ENST00000571761 ENST00000577107 ENST00000575965 ENST00000571164

Gene Information

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol steroids and other lipids. This protein localizes to the mitochondrial inner membrane and is involved in the conversion of progesterone to cortisol in the adrenal cortex. Mutations in this gene cause congenital adrenal hyperplasia due to 11-beta-hydroxylase deficiency. Transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   CYP11B1
Gene Name:   cytochrome P450, family 11, subfamily B, polypeptide 1
Aliases:   CPN1, CYP11B, DKFZp686B05283, FHI, FLJ36771, P450C11
RefSeq:   NC_000008.10 NG_007954.1 NT_008046.16
Ensembl:   ENSG00000160882
Entrez:   1584
Chromosome Mapping:   8q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997900
y-intercept 36.490000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download