PrimePCR™ SYBR® Green Assay: HDAC2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0055364


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PrimePCR™ PreAmp for SYBR® Green Assay: HDAC2, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: HDAC2, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0048104
Assay Design:		exonic
Chromosome Location:		6:114281081-114281182question
Amplicon Length:		72
Splice Variants Targeted:		ENST00000519065 ENST00000519108 ENST00000368632 ENST00000425835 ENST00000523628 ENST00000521610 ENST00000522371 ENST00000521163 ENST00000518690 ENST00000523240 ENST00000520895 ENST00000524334 ENST00000398283

Gene Information

This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A H2B H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins including YY1 a mammalian zinc-finger transcription factor. Thus it plays an important role in transcriptional regulation cell cycle progression and developmental events. Alternative splicing results in multiple transcript variants. [provided by RefSeq Apr 2010]

Gene Symbol:		HDAC2
Gene Name:		histone deacetylase 2
Aliases:		HD2, RPD3, YAF1
RefSeq:		NC_000006.11 NT_025741.15
Ensembl:		ENSG00000196591
Entrez:		3066
Chromosome Mapping:		6q21

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999600
y-intercept	36.210000
Efficiency	95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.