PrimePCR™ SYBR® Green Assay: Hist1h4b, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qMmuCEP0043457


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PrimePCR™ PreAmp for SYBR® Green Assay: Hist1h4b, Mouse
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: Hist1h4b, Mouse
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qMmuCED0041240
Assay Design:		exonic
Chromosome Location:		13:23757024-23757184question
Amplicon Length:		131
Splice Variants Targeted:		ENSMUST00000055993 ENSMUST00000120876 ENSMUST00000069044 ENSMUST00000079618 ENSMUST00000075290 ENSMUST00000087714 ENSMUST00000102983 ENSMUST00000104941 ENSMUST00000102979 ENSMUST00000102977 ENSMUST00000102972 ENSMUST00000102971 ENSMUST00000102967 ENSMUST00000102965 ENSMUST00000102964 ENSMUST00000009138 ENSMUST00000119274 ENSMUST00000061835 ENSMUST00000177086 ENSMUST00000078259 ENSMUST00000089217 ENSMUST00000001989 ENSMUST00000039734 ENSMUST00000160896 ENSMUST00000015271 ENSMUST00000023068 ENSMUS

Gene Information

Description Not Available

Gene Symbol:		Hist1h4b
Gene Name:		Histone cluster 1, H4b
Aliases:		Not Available
RefSeq:		NC_000079.6 NT_039578.8
Ensembl:		ENSMUSG00000069266
Entrez:		326620
Chromosome Mapping:		13 A2-A3\|13

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Mouse Reference Total RNA

Summary Data:

R²	0.999400
y-intercept	37.070000
Efficiency	96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.