PrimePCR™ SYBR® Green Assay: Cyp2c50, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qMmuCEP0062803

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Cyp2c50, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Cyp2c50, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0061654
Assay Design:   exonic
Chromosome Location:   19:40090581-40090947question
Amplicon Length:   88
Splice Variants Targeted:   ENSMUST00000079081 ENSMUST00000106770 ENSMUST00000030785 ENSMUST00000105659 ENSMUST00000105658 ENSMUST00000070018 ENSMUST00000105657 ENSMUST00000080042 ENSMUST00000084114 ENSMUST00000105655 ENSMUST00000105656 ENSMUST00000103196 ENSMUST00000105654 ENSMUST00000135185 ENSMUST00000087236 ENSMUST00000087234 ENSMUST00000049178 ENSMUST00000048959 ENSMUST00000068094 ENSMUST00000080171 ENSMUST00000045779 ENSMUST00000031729

Gene Information

Description Not Available

Gene Symbol:   Cyp2c50
Gene Name:   Cytochrome P450, family 2, subfamily c, polypeptide 50
Aliases:   AI266900
RefSeq:   NC_000085.6 NT_039687.8
Ensembl:   ENSMUSG00000054827
Entrez:   107141
Chromosome Mapping:   19 C3|19

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.000000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download