PrimePCR™ SYBR® Green Assay: Bcl2l1, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.


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PrimePCR™ PreAmp for SYBR® Green Assay: Bcl2l1, Rat
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: Bcl2l1, Rat
Reaction: 4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qRnoCED0051178
Assay Design:		Exonic
Chromosome Location:		1:173950499-173950644question
Amplicon Length:		116
Splice Variants Targeted:		ENSRNOT00000072778 ENSRNOT00000002550 ENSRNOT00000023036 ENSRNOT00000008963 ENSRNOT00000007625 ENSRNOT00000022951 ENSRNOT00000028295 ENSRNOT00000066182 ENSRNOT00000024954

Gene Information

inhibits neuronal apoptosis; may provide neuroprotection against ischemic brian injury [RGD, Feb 2006]

Gene Symbol:		Bcl2l1
Gene Name:		Bcl-2-like protein 1 isoform 3
Aliases:		Not Available
RefSeq:		NM_001033670 NM_001033671 NM_001033672 NM_031535
Ensembl:		ENSRNOG00000018503
Entrez:		24888
Chromosome Mapping:		3q41.2

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Rat Reference Total RNA

Summary Data:

R²	0.999800
y-intercept	37.290000
Efficiency	93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.