PrimePCR™ SYBR® Green Assay: PFN2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0025723


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PrimePCR™ PreAmp for SYBR® Green Assay: PFN2, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: PFN2, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0007890
Assay Design:		Exonic
Chromosome Location:		3:149686205-149686333question
Amplicon Length:		99
Splice Variants Targeted:		ENST00000452853 ENST00000239940 ENST00000423691 ENST00000481767 ENST00000494827 ENST00000490975 ENST00000497148 ENST00000475518 ENST00000481275 ENST00000498307 ENST00000489155 ENST00000461930 ENST00000461868

Gene Information

The protein encoded by this gene is a ubiquitous actin monomer-binding protein belonging to the profilin family. It is thought to regulate actin polymerization in response to extracellular signals. There are two alternatively spliced transcript variants encoding different isoforms described for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:		PFN2
Gene Name:		profilin 2
Aliases:		D3S1319E, PFL
RefSeq:		NC_000003.11 NT_005612.16
Ensembl:		ENSG00000070087
Entrez:		5217
Chromosome Mapping:		3q25.1

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999500
y-intercept	34.170000
Efficiency	98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.