PrimePCR™ SYBR® Green Assay: CDKN2B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0052653

List Price:    $179.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CDKN2B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CDKN2B, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0045392
Assay Design:   exonic
Chromosome Location:   9:22008981-22009095question
Amplicon Length:   85
Splice Variants Targeted:   ENST00000276925 ENST00000380142

Gene Information

This gene lies adjacent to the tumor suppressor gene CDKN2A in a region that is frequently mutated and deleted in a wide variety of tumors. This gene encodes a cyclin-dependent kinase inhibitor which forms a complex with CDK4 or CDK6 and prevents the activation of the CDK kinases thus the encoded protein functions as a cell growth regulator that controls cell cycle G1 progression. The expression of this gene was found to be dramatically induced by TGF beta which suggested its role in the TGF beta induced growth inhibition. Two alternatively spliced transcript variants of this gene which encode distinct proteins have been reported. [provided by RefSeq Jul 2008]

Gene Symbol:   CDKN2B
Gene Name:   cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4)
Aliases:   CDK4I, INK4B, MTS2, P15, TP15, p15INK4b
RefSeq:   NC_000009.11 NT_008413.18 NG_023297.1
Ensembl:   ENSG00000147883
Entrez:   1030
Chromosome Mapping:   9p21

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