PrimePCR™ SYBR® Green Assay: WRN, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0053562

List Price:    $179.00
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PrimePCR™ PreAmp for SYBR® Green Assay: WRN, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: WRN, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0046301
Assay Design:   exonic
Chromosome Location:   8:31030650-31030782question
Amplicon Length:   103
Splice Variants Targeted:   ENST00000298139

Gene Information

This gene encodes a member of the RecQ subfamily and the DEAH (Asp-Glu-Ala-His) subfamily of DNA and RNA helicases. DNA helicases are involved in many aspects of DNA metabolism including transcription replication recombination and repair. This protein contains a nuclear localization signal in the C-terminus and shows a predominant nucleolar localization. It possesses an intrinsic 3' to 5' DNA helicase activity and is also a 3' to 5' exonuclease. Based on interactions between this protein and Ku70/80 heterodimer in DNA end processing this protein may be involved in the repair of double strand DNA breaks. Defects in this gene are the cause of Werner syndrome an autosomal recessive disorder characterized by premature aging. [provided by RefSeq Jul 2008]

Gene Symbol:   WRN
Gene Name:   Werner syndrome, RecQ helicase-like
Aliases:   DKFZp686C2056, RECQ3, RECQL2, RECQL3
RefSeq:   NC_000008.10 NG_008870.1 NT_167187.1
Ensembl:   ENSG00000165392
Entrez:   7486
Chromosome Mapping:   8p12

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