PrimePCR™ SYBR® Green Assay: DDX39B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0057919

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: DDX39B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: DDX39B, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0056814
Assay Design:   exonic
Chromosome Location:   6:31498594-31498703question
Amplicon Length:   80
Splice Variants Targeted:   ENST00000448800 ENST00000426766 ENST00000420337 ENST00000437943 ENST00000412386 ENST00000552820 ENST00000450987 ENST00000431360 ENST00000430784 ENST00000455400 ENST00000550217 ENST00000446341 ENST00000412330 ENST00000448296 ENST00000445218 ENST00000433293 ENST00000548614 ENST00000376177 ENST00000458640 ENST00000396172 ENST00000417023 ENST00000417556 ENST00000415382 ENST00000430703 ENST00000416863 ENST00000413678 ENST00000414440 ENST00000428107 ENST00000547489 ENST00000453488 ENST00000441425 ENST

Gene Information

This gene encodes a member of the DEAD box family of RNA-dependent ATPases that mediate ATP hydrolysis during pre-mRNA splicing. The encoded protein is an essential splicing factor required for association of U2 small nuclear ribonucleoprotein with pre-mRNA and it also plays an important role in mRNA export from the nucleus to the cytoplasm. This gene belongs to a cluster of genes localized in the vicinity of the genes encoding tumor necrosis factor alpha and tumor necrosis factor beta. These genes are all within the human major histocompatibility complex class III region. Mutations in this gene may be associated with rheumatoid arthritis. Alternative splicing results in multiple transcript variants. Related pseudogenes have been identified on both chromosomes 6 and 11. Read-through transcription also occurs between this gene and the upstream ATP6V1G2 (ATPase H+ transporting lysosomal 13kDa V1 subunit G2) gene. [provided by RefSeq Feb 2011]

Gene Symbol:   DDX39B
Gene Name:   DEAD (Asp-Glu-Ala-Asp) box polypeptide 39B
Aliases:   BAT1, D6S81E, UAP56
RefSeq:   NC_000006.11 NG_012344.1 NT_007592.15 NT_113891.2 NT_167244.1 NT_167245.1 NT_167246.1 NT_167247.1 NT_167248.1 NT_167249.1
Ensembl:   ENSG00000198563
Entrez:   7919
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.620000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download