PrimePCR™ SYBR® Green Assay: PDE4A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0026210


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PrimePCR™ PreAmp for SYBR® Green Assay: PDE4A, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: PDE4A, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0006541
Assay Design:		Intron-spanning
Chromosome Location:		19:10572636-10574592question
Amplicon Length:		134
Splice Variants Targeted:		ENST00000380702 ENST00000352831 ENST00000293683 ENST00000440014 ENST00000344979 ENST00000380686 ENST00000419866

Gene Information

The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family and PDE4 subfamily. This PDE hydrolyzes the second messenger cAMP which is a regulator and mediator of a number of cellular responses to extracellular signals. Thus by regulating the cellular concentration of cAMP this protein plays a key role in many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq Jul 2011]

Gene Symbol:		PDE4A
Gene Name:		phosphodiesterase 4A, cAMP-specific
Aliases:		DPDE2, PDE4, PDE46
RefSeq:		NC_000019.9 NT_011295.11
Ensembl:		ENSG00000065989
Entrez:		5141
Chromosome Mapping:		19p13.2

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.998900
y-intercept	34.720000
Efficiency	101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.