PrimePCR™ SYBR® Green Assay: Psme2, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qMmuCEP0043659


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PrimePCR™ PreAmp for SYBR® Green Assay: Psme2, Mouse
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: Psme2, Mouse
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qMmuCED0041443
Assay Design:		exonic
Chromosome Location:		14:55587654-55588060question
Amplicon Length:		77
Splice Variants Targeted:		ENSMUST00000047480 ENSMUST00000072800 ENSMUST00000024810 ENSMUST00000167591 ENSMUST00000092070 ENSMUST00000161807 ENSMUST00000111378 ENSMUST00000020586 ENSMUST00000092915 ENSMUST00000071972 ENSMUST00000081921 ENSMUST00000106239 ENSMUST00000062306 ENSMUST00000104958 ENSMUST00000178124 ENSMUST00000099557 ENSMUST00000110853 ENSMUST00000084787

Gene Information

Description Not Available

Gene Symbol:		Psme2
Gene Name:		Proteasome (prosome, macropain) 28 subunit, beta
Aliases:		AA589371, AI788882, PA28b
RefSeq:		NC_000080.6 NT_039606.8
Ensembl:		ENSMUSG00000079197
Entrez:		19188
Chromosome Mapping:		14 C2-D1\|14 22.5 cM

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Mouse Reference Total RNA

Summary Data:

R²	0.999900
y-intercept	37.890000
Efficiency	92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.