PrimePCR™ SYBR® Green Assay: Map3k4, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qMmuCIP0043683


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PrimePCR™ PreAmp for SYBR® Green Assay: Map3k4, Mouse
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: Map3k4, Mouse
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qMmuCID0041467
Assay Design:		Intron-spanning
Chromosome Location:		17:12256807-12257956question
Amplicon Length:		65
Splice Variants Targeted:		ENSMUST00000163257 ENSMUST00000091459 ENSMUST00000099358 ENSMUST00000121808 ENSMUST00000121888 ENSMUST00000118313 ENSMUST00000121774 ENSMUST00000167161 ENSMUST00000166044 ENSMUST00000089058 ENSMUST00000115691 ENSMUST00000025186 ENSMUST00000169397 ENSMUST00000118455

Gene Information

Description Not Available

Gene Symbol:		Map3k4
Gene Name:		Mitogen-activated protein kinase kinase kinase 4
Aliases:		Not Available
RefSeq:		Not Available
Ensembl:		ENSMUSG00000079716
Chromosome Mapping:		Not Available

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Mouse Reference Total RNA

Summary Data:

R²	0.999700
y-intercept	37.630000
Efficiency	91

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.