PrimePCR™ SYBR® Green Assay: Cttn, Rat

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.


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PrimePCR™ PreAmp for SYBR® Green Assay: Cttn, Rat
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: Cttn, Rat
Reaction: 4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qRnoCID0012855
Assay Design:		intron_spanning
Chromosome Location:		1:222163085-222164236question
Amplicon Length:		96
Splice Variants Targeted:		ENSRNOT00000023861 ENSRNOT00000033827 ENSRNOT00000033919 ENSRNOT00000041034 ENSRNOT00000011290 ENSRNOT00000059974 ENSRNOT00000041988 ENSRNOT00000011371 ENSRNOT00000071529 ENSRNOT00000066774 ENSRNOT00000020768 ENSRNOT00000010895 ENSRNOT00000051590 ENSRNOT00000040845 ENSRNOT00000030344 ENSRNOT00000015181 ENSRNOT00000026448 ENSRNOT00000030037 ENSRNOT00000027836 ENSRNOT00000028283 ENSRNOT00000047669 ENSRNOT00000054859 ENSRNOT00000020763

Gene Information

component of clathrin-coated pits; involved in receptor-mediated endocytosis [RGD, Feb 2006]

Gene Symbol:		Cttn
Gene Name:		Cortactin isoform B
Aliases:		Not Available
RefSeq:		NM_021868
Ensembl:		ENSRNOG00000050994
Entrez:		60465
Chromosome Mapping:		1q41-q42

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Rat Reference Total RNA

Summary Data:

R²	0.996800
y-intercept	37.850000
Efficiency	91

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.