PrimePCR™ SYBR® Green Assay: NDE1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0030704

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: NDE1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: NDE1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0016618
Assay Design:   Intron-spanning
Chromosome Location:   16:15758674-15761265question
Amplicon Length:   138
Splice Variants Targeted:   ENST00000396355 ENST00000396354 ENST00000342673 ENST00000396353

Gene Information

This gene encodes a member of the nuclear distribution E (NudE) family of proteins. The encoded protein is localized at the centrosome and interacts with other centrosome components as part of a multiprotein complex that regulates dynein function. This protein plays an essential role in microtubule organization mitosis and neuronal migration. Mutations in this gene cause lissencephaly 4 a disorder characterized by lissencephaly severe brain atrophy microcephaly and severe mental retardation. Alternative splicing results in multiple transcript variants. [provided by RefSeq Mar 2012]

Gene Symbol:   NDE1
Gene Name:   nudE nuclear distribution gene E homolog 1 (A. nidulans)
Aliases:   FLJ20101, HOM-TES-87, NUDE, NUDE1
RefSeq:   NG_021407.1 NC_000016.9 NG_009299.1 NG_021210.1 NT_010393.16
Ensembl:   ENSG00000072864
Entrez:   54820
Chromosome Mapping:   16p13.11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 34.900000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download