PrimePCR™ lncRNA SYBR® Green Assay:SPRY4-IT1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression.

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qhsaLEP0212752. May amplify unspliced transcripts of an antisense gene.

List Price:    $169.00
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PrimePCR™ IncRNA PreAmp for SYBR® Green Assay: SPRY4-IT1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ IncRNA Template for SYBR® Green Assay: SPRY4-IT1, Human
Reaction:     4 billion copies/200 µl desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   lncRNA Expression
Unique Assay ID:   qhsaLED0135512
Assay Design:   exonic
Chromosome Location:   chr5:142317886-142318027question
Amplicon Length:   112
Refseq Transcripts Amplified:   NR_131221.1
Splice Variants Targeted:   ENST00000630558
LNCipedia Splice Variants Targeted:   lnc-FGF1-9:1

Gene Information

Not Available

Gene Symbol:   SPRY4-IT1
Gene Name:   SPRY4 intronic transcript 1 [Source:HGNC Symbol;Acc:HGNC:42394]
Aliases:   SPRIGHTLY
RefSeq:   Not Available
Ensembl:   ENSG00000281881
LNCipedia:   lnc-FGF1-9
Entrez:   100642175
Chromosome Mapping:   5q31.3
Gene Strand:   Reverse strand

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.259998
Efficiency 1.94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download