PrimePCR™ SYBR® Green Assay: Vegfa, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qMmuCEP0042478


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PrimePCR™ PreAmp for SYBR® Green Assay: Vegfa, Mouse
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: Vegfa, Mouse
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qMmuCED0040260
Assay Design:		exonic
Chromosome Location:		17:46024444-46025360question
Amplicon Length:		60
Splice Variants Targeted:		ENSMUST00000024747 ENSMUST00000071648 ENSMUST00000142351 ENSMUST00000113520 ENSMUST00000113519 ENSMUST00000167860

Gene Information

This gene product is a member of the PDGF/VEGF growth factor family. It is a mitogen that specifically acts on endothelial cells and has various effects including mediating increased vascular permeability inducing angiogenesis vasculogenesis endothelial cell growth promoting cell migration and inhibiting apoptosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Also alternative translation initiation from non-AUG (CUG) and AUG start sites in some transcript variants give rise to additional isoforms. [provided by RefSeq Jul 2008]

Gene Symbol:		Vegfa
Gene Name:		Vascular endothelial growth factor A
Aliases:		Vegf, Vegf120, Vegf164, Vegf188, Vpf
RefSeq:		NC_000083.6 NT_039649.8
Ensembl:		ENSMUSG00000023951
Entrez:		22339
Chromosome Mapping:		17 C\|17 24.2 cM

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Mouse Reference Total RNA

Summary Data:

R²	0.999200
y-intercept	35.710000
Efficiency	101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.