PrimePCR™ SYBR® Green Assay: Sep15, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qMmuCEP0057206

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: Sep15, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Sep15, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCED0048780
Assay Design:   exonic
Chromosome Location:   3:144597063-144597198question
Amplicon Length:   106
Splice Variants Targeted:   ENSMUST00000082437 ENSMUST00000106211

Gene Information

This gene encodes a selenoprotein which contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the UGA codon that normally signals translation termination. The 3' UTR of selenoprotein genes have a common stem-loop structure the sec insertion sequence (SECIS) that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. The exact function of this protein is not known however it has been found to associate with UDP-glucose:glycoprotein glucosyltransferase (UGTR) an endoplasmic reticulum(ER)-resident protein which is involved in the quality control of protein folding. The association with UGTR retains this selenoprotein in the ER where it may play a role in protein folding. [provided by RefSeq Jul 2008]

Gene Symbol:   Sep15
Gene Name:   Selenoprotein
Aliases:   9430015P09Rik
RefSeq:   NC_000069.6 NT_039240.8
Ensembl:   ENSMUSG00000037072
Entrez:   93684
Chromosome Mapping:   3|3 H3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Mouse Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.780000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download