PrimePCR™ SYBR® Green Assay: Polr1d, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Recommended - best efficiency; Same primer pair as used in probe assay qMmuCEP0062935


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PrimePCR™ PreAmp for SYBR® Green Assay: Polr1d, Mouse
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: Polr1d, Mouse
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qMmuCED0061786
Assay Design:		exonic
Chromosome Location:		5:147077620-147078560question
Amplicon Length:		70
Splice Variants Targeted:		ENSMUST00000053871 ENSMUST00000167671 ENSMUST00000034949 ENSMUST00000117849 ENSMUST00000147495 ENSMUST00000034611 ENSMUST00000138356 ENSMUST00000134465 ENSMUST00000148344 ENSMUST00000115451 ENSMUST00000030763 ENSMUST00000115452 ENSMUST00000115450 ENSMUST00000036161 ENSMUST00000154364 ENSMUST00000149309 ENSMUST00000025728 ENSMUST00000105682 ENSMUST00000118326 ENSMUST00000119498 ENSMUST00000061789 ENSMUST00000093132 ENSMUST00000109923 ENSMUST00000021950 ENSMUST00000109921 ENSMUST00000100487 ENSMUS

Gene Information

Description Not Available

Gene Symbol:		Polr1d
Gene Name:		Polymerase (RNA) I polypeptide D
Aliases:		1110003G10Rik, 16kDa, AU018636, C81327, Rpo1-3, mRPA16
RefSeq:		NC_000071.6 NT_039324.8
Ensembl:		ENSMUSG00000029642
Entrez:		20018
Chromosome Mapping:		5 G3\|5

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Mouse Reference Total RNA

Summary Data:

R²	0.999800
y-intercept	36.110000
Efficiency	101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.