PrimePCR™ SYBR® Green Assay: ELN, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0057837

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ELN, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ELN, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0056732
Assay Design:   exonic
Chromosome Location:   7:73483522-73483671question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000360537 ENST00000354765 ENST00000367572 ENST00000367571 ENST00000444202 ENST00000429150 ENST00000392309 ENST00000416623 ENST00000437412 ENST00000392307 ENST00000320631 ENST00000359393 ENST00000326446 ENST00000334746 ENST00000554565 ENST00000553975 ENST00000393170 ENST00000405844 ENST00000374155 ENST00000344321 ENST00000441828 ENST00000282406 ENST00000418390 ENST00000542504 ENST00000335681

Gene Information

This gene encodes a protein that is one of the two components of elastic fibers. The encoded protein is rich in hydrophobic amino acids such as glycine and proline which form mobile hydrophobic regions bounded by crosslinks between lysine residues. Deletions and mutations in this gene are associated with supravalvular aortic stenosis (SVAS) and autosomal dominant cutis laxa. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   ELN
Gene Name:   elastin
Aliases:   FLJ38671, FLJ43523, SVAS, WBS, WS
RefSeq:   NC_000007.13 NG_009261.1 NT_007933.15
Ensembl:   ENSG00000049540
Entrez:   2006
Chromosome Mapping:   7q11.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997600
y-intercept 37.400000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download