PrimePCR™ SYBR® Green Assay: CYP51A1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0040638

List Price:    $179.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CYP51A1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CYP51A1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0037915
Assay Design:   Intron-spanning
Chromosome Location:   7:91746498-91752477question
Amplicon Length:   139
Splice Variants Targeted:   ENST00000003100 ENST00000450723 ENST00000422867

Gene Information

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol steroids and other lipids. This endoplasmic reticulum protein participates in the synthesis of cholesterol by catalyzing the removal of the 14alpha-methyl group from lanosterol. Homologous genes are found in all three eukaryotic phyla fungi plants and animals suggesting that this is one of the oldest cytochrome P450 genes. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Mar 2009]

Gene Symbol:   CYP51A1
Gene Name:   cytochrome P450, family 51, subfamily A, polypeptide 1
Aliases:   CP51, CYP51, CYPL1, LDM, P450-14DM, P450L1
RefSeq:   NC_000007.13 NG_007968.1 NG_011623.1 NT_007933.15
Ensembl:   ENSG00000001630
Entrez:   1595
Chromosome Mapping:   7q21.2

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