PrimePCR™ SYBR® Green Assay: NFKBIB, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0040370

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: NFKBIB, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: NFKBIB, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0037647
Assay Design:   exonic
Chromosome Location:   19:39396054-39396160question
Amplicon Length:   77
Splice Variants Targeted:   ENST00000576510 ENST00000572515 ENST00000575359 ENST00000313582 ENST00000392079

Gene Information

The protein encoded by this gene belongs to the NF-kappa-B inhibitor family which inhibit NF-kappa-B by complexing with and trapping it in the cytoplasm. Phosphorylation of serine residues on these proteins by kinases marks them for destruction via the ubiquitination pathway thereby allowing activation of the NF-kappa-B which translocates to the nucleus to function as a transcription factor. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq Jul 2011]

Gene Symbol:   NFKBIB
Gene Name:   nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, beta
Aliases:   IKBB, TRIP9
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000104825
Entrez:   4793
Chromosome Mapping:   19q13.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.410000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download