PrimePCR™ SYBR® Green Assay: GRIN1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: GRIN1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: GRIN1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0010984
Assay Design:   Intron-spanning
Chromosome Location:   9:140051437-140052875question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000371561 ENST00000350902 ENST00000371550 ENST00000371546 ENST00000371555 ENST00000371553 ENST00000371559 ENST00000371560 ENST00000315048

Gene Information

The protein encoded by this gene is a critical subunit of N-methyl-D-aspartate receptors members of the glutamate receptor channel superfamily which are heteromeric protein complexes with multiple subunits arranged to form a ligand-gated ion channel. These subunits play a key role in the plasticity of synapses which is believed to underlie memory and learning. Cell-specific factors are thought to control expression of different isoforms possibly contributing to the functional diversity of the subunits. Alternatively spliced transcript variants have been described. [provided by RefSeq Jul 2008]

Gene Symbol:   GRIN1
Gene Name:   glutamate receptor, ionotropic, N-methyl D-aspartate 1
Aliases:   NMDA1, NMDAR1, NR1
RefSeq:   NC_000009.11 NT_024000.16 NG_011507.1
Ensembl:   ENSG00000176884
Entrez:   2902
Chromosome Mapping:   9q34.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997400
y-intercept 34.540000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download