PrimePCR™ SYBR® Green Assay: VIM, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: VIM, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: VIM, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0012604
Assay Design:   Intron-spanning
Chromosome Location:   10:17272649-17275636question
Amplicon Length:   82
Splice Variants Targeted:   ENST00000224237 ENST00000421459 ENST00000544301 ENST00000545533

Gene Information

This gene encodes a member of the intermediate filament family. Intermediate filamentents along with microtubules and actin microfilaments make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape integrity of the cytoplasm and stabilizing cytoskeletal interactions. It is also involved in the immune response and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment migration and cell signaling. Mutations in this gene causes a dominant pulverulent cataract.[provided by RefSeq Jun 2009]

Gene Symbol:   VIM
Gene Name:   vimentin
Aliases:   FLJ36605
RefSeq:   NC_000010.10 NG_012413.1 NT_008705.16
Ensembl:   ENSG00000026025
Entrez:   7431
Chromosome Mapping:   10p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 34.930000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download