PrimePCR™ SYBR® Green Assay: Adarb1, Mouse

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: Adarb1, Mouse
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: Adarb1, Mouse
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qMmuCID0023100
Assay Design:   Intron-spanning
Chromosome Location:   10:77322539-77325937question
Amplicon Length:   87
Splice Variants Targeted:   ENSMUST00000020496 ENSMUST00000098374 ENSMUST00000105406

Gene Information

This gene encodes a double-stranded-RNA-specific adenosine deaminase that is involved in editing pre-mRNAs by site-specific conversion of adenosine (A) to inosine (I). Substrates for this enzyme include ionotropic glutamate receptors (GluR2-6) and serotonin receptor (5HT2C). Studies in rodents have shown that this protein can modify its own pre-mRNA by A->I editing to create a novel acceptor splice site alternative splicing to which results in down regulation of its protein expression. Additional splicing events result in transcript variants encoding different isoforms. [provided by RefSeq Jul 2008]

Gene Symbol:   Adarb1
Gene Name:   Adenosine deaminase, RNA-specific, B1
Aliases:   1700057H01Rik, AW124433, AW558573, Adar2, BB220382, D10Bwg0447e, Red1
RefSeq:   NC_000076.6 NT_039500.8
Ensembl:   ENSMUSG00000020262
Entrez:   110532
Chromosome Mapping:   10 C|10 41.4 cM

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