PrimePCR™ SYBR® Green Assay: ACTA1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: ACTA1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ACTA1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0005405
Assay Design:   Exonic
Chromosome Location:   1:229568017-229568106question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000366684 ENST00000366682

Gene Information

The product encoded by this gene belongs to the actin family of proteins which are highly conserved proteins that play a role in cell motility structure and integrity. Alpha beta and gamma actin isoforms have been identified with alpha actins being a major constituent of the contractile apparatus while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3 congenital myopathy with excess of thin myofilaments congenital myopathy with cores and congenital myopathy with fiber-type disproportion diseases that lead to muscle fiber defects. [provided by RefSeq Jul 2008]

Gene Symbol:   ACTA1
Gene Name:   actin, alpha 1, skeletal muscle
Aliases:   ACTA, ASMA, CFTD, CFTD1, CFTDM, MPFD, NEM1, NEM2, NEM3
RefSeq:   NC_000001.10 NG_006672.1 NT_167186.1
Ensembl:   ENSG00000143632
Entrez:   58
Chromosome Mapping:   1q42.13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.140000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download