PrimePCR™ SYBR® Green Assay: COL4A2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0051838


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PrimePCR™ PreAmp for SYBR® Green Assay: COL4A2, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: COL4A2, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0044576
Assay Design:		exonic
Chromosome Location:		13:110960446-111077081question
Amplicon Length:		77
Splice Variants Targeted:		ENST00000400163 ENST00000360467

Gene Information

This gene encodes one of the six subunits of type IV collagen the major structural component of basement membranes. The C-terminal portion of the protein known as canstatin is an inhibitor of angiogenesis and tumor growth. Like the other members of the type IV collagen gene family this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. [provided by RefSeq Jul 2008]

Gene Symbol:		COL4A2
Gene Name:		collagen, type IV, alpha 2
Aliases:		DKFZp686I14213, FLJ22259
RefSeq:		NG_011544.1 NC_000013.10 NT_009952.14
Ensembl:		ENSG00000134871
Entrez:		1284
Chromosome Mapping:		13q34

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999800
y-intercept	35.810000
Efficiency	97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.