PrimePCR™ SYBR® Green Assay: NME1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0056013

List Price:    $179.00
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PrimePCR™ PreAmp for SYBR® Green Assay: NME1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: NME1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Wet Lab Validated

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0048753
Assay Design:   exonic
Chromosome Location:   17:49233036-49237343question
Amplicon Length:   79
Splice Variants Targeted:   ENST00000393196 ENST00000336097 ENST00000480143 ENST00000511355 ENST00000013034 ENST00000456492 ENST00000393193 ENST00000376392 ENST00000555572

Gene Information

This gene (NME1) was identified because of its reduced mRNA transcript levels in highly metastatic cells. Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by this gene) and 'B' (encoded by NME2) isoforms. Mutations in this gene have been identified in aggressive neuroblastomas. Two transcript variants encoding different isoforms have been found for this gene. Co-transcription of this gene and the neighboring downstream gene (NME2) generates naturally-occurring transcripts (NME1-NME2) which encodes a fusion protein comprised of sequence sharing identity with each individual gene product. [provided by RefSeq Jul 2008]

Gene Symbol:   NME1
Gene Name:   non-metastatic cells 1, protein (NM23A) expressed in
Aliases:   AWD, GAAD, NB, NBS, NDKA, NDPK-A, NDPKA, NM23, NM23-H1
RefSeq:   NC_000017.10 NT_010783.15 NG_021169.1
Ensembl:   ENSG00000239672
Entrez:   4830
Chromosome Mapping:   17q21.3

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