PrimePCR™ SYBR® Green Assay: ITGA3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0057746


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PrimePCR™ PreAmp for SYBR® Green Assay: ITGA3, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: ITGA3, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0056641
Assay Design:		exonic
Chromosome Location:		17:48156197-48156442question
Amplicon Length:		67
Splice Variants Targeted:		ENST00000320031 ENST00000007722 ENST00000506827

Gene Information

The protein encoded by this gene belongs to the family of integrins. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain and function as cell surface adhesion molecules. This gene encodes alpha 3 subunit which undergoes post-translational cleavage in the extracellular domain to yield disulfide-linked light and heavy chains that join with beta 1 subunit to form an integrin that interacts with many extracellular-matrix proteins. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq Oct 2008]

Gene Symbol:		ITGA3
Gene Name:		integrin, alpha 3 (antigen CD49C, alpha 3 subunit of VLA-3 receptor)
Aliases:		CD49C, FLJ34631, FLJ34704, GAP-B3, GAPB3, MSK18, VCA-2, VL3A, VLA3a
RefSeq:		NC_000017.10 NT_010783.15
Ensembl:		ENSG00000005884
Entrez:		3675
Chromosome Mapping:		17q21.33

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999900
y-intercept	36.950000
Efficiency	97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.