PrimePCR™ SYBR® Green Assay: ITGA6, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.


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PrimePCR™ PreAmp for SYBR® Green Assay: ITGA6, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: ITGA6, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0007188
Assay Design:		Intron-spanning
Chromosome Location:		2:173356166-173362776question
Amplicon Length:		130
Splice Variants Targeted:		ENST00000409532 ENST00000264107 ENST00000409080 ENST00000442250 ENST00000458358 ENST00000416789 ENST00000264106 ENST00000375221 ENST00000343713

Gene Information

The ITGA6 protein product is the integrin alpha chain alpha 6. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example alpha 6 may combine with beta 4 in the integrin referred to as TSP180 or with beta 1 in the integrin VLA-6. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:		ITGA6
Gene Name:		integrin, alpha 6
Aliases:		CD49f, DKFZp686J01244, FLJ18737, ITGA6B, VLA-6
RefSeq:		NC_000002.11 NT_005403.17 NG_008853.1
Ensembl:		ENSG00000091409
Entrez:		3655
Chromosome Mapping:		2q31.1

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999200
y-intercept	34.440000
Efficiency	109

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.