PrimePCR™ SYBR® Green Assay: CDC14A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: CDC14A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CDC14A, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0008400
Assay Design:   Intron-spanning
Chromosome Location:   1:100889781-100905538question
Amplicon Length:   98
Splice Variants Targeted:   ENST00000455467 ENST00000361544 ENST00000370124 ENST00000336454 ENST00000544534 ENST00000370125 ENST00000542213

Gene Information

The protein encoded by this gene is a member of the dual specificity protein tyrosine phosphatase family. It is highly similar to Saccharomyces cerevisiae Cdc14 a protein tyrosine phosphatase involved in the exit of cell mitosis and initiation of DNA replication suggesting a role in cell cycle control. This protein has been shown to interact with and dephosphorylate tumor suppressor protein p53 and is thought to regulate the function of p53. Alternative splicing of this gene results in several transcript variants encoding distinct isoforms. [provided by RefSeq Jul 2008]

Gene Symbol:   CDC14A
Gene Name:   CDC14 cell division cycle 14 homolog A (S. cerevisiae)
Aliases:   cdc14, hCDC14
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000079335
Entrez:   8556
Chromosome Mapping:   1p21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.995600
y-intercept 35.870000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download