PrimePCR™ SYBR® Green Assay: GNAI2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028320

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: GNAI2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: GNAI2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0012136
Assay Design:   Intron-spanning
Chromosome Location:   3:50290560-50293661question
Amplicon Length:   65
Splice Variants Targeted:   ENST00000422163 ENST00000313601 ENST00000440628 ENST00000451956 ENST00000540560 ENST00000266027 ENST00000536647

Gene Information

The protein encoded by this gene is an alpha subunit of guanine nucleotide binding proteins (G proteins). The encoded protein contains the guanine nucleotide binding site and is involved in the hormonal regulation of adenylate cyclase. Several transcript variants encoding different isoforms have been detected for this gene but the full-length nature of only two are known so far. [provided by RefSeq Oct 2009]

Gene Symbol:   GNAI2
Gene Name:   guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 2
Aliases:   GIP, GNAI2B, H_LUCA15.1, H_LUCA16.1
RefSeq:   NC_000003.11 NT_022517.18 NG_016002.1
Ensembl:   ENSG00000114353
Entrez:   2771
Chromosome Mapping:   3p21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 34.770000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download