PrimePCR™ SYBR® Green Assay: CCT6B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0031151

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CCT6B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CCT6B, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0017299
Assay Design:   Intron-spanning
Chromosome Location:   17:33278980-33281517question
Amplicon Length:   136
Splice Variants Targeted:   ENST00000421975 ENST00000314144 ENST00000436961

Gene Information

This gene encodes a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT) also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins including actin and tubulin. Alternate transcriptional splice variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2010]

Gene Symbol:   CCT6B
Gene Name:   chaperonin containing TCP1, subunit 6B (zeta 2)
Aliases:   CCT-zeta-2, CCTZ-2, Cctz2, TCP-1-zeta-2, TSA303
RefSeq:   NC_000017.10 NT_010799.15
Ensembl:   ENSG00000132141
Entrez:   10693
Chromosome Mapping:   17q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.330000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download