PrimePCR™ SYBR® Green Assay: SNAP91, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCIP0040616


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PrimePCR™ PreAmp for SYBR® Green Assay: SNAP91, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: SNAP91, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCID0037893
Assay Design:		Intron-spanning
Chromosome Location:		6:84372081-84417524question
Amplicon Length:		181
Splice Variants Targeted:		ENST00000521485 ENST00000369694 ENST00000195649 ENST00000439399 ENST00000521743 ENST00000520302 ENST00000520213 ENST00000521931 ENST00000519779 ENST00000518309 ENST00000369690 ENST00000523484 ENST00000428679 ENST00000437520

Gene Information

Description Not Available

Gene Symbol:		SNAP91
Gene Name:		synaptosomal-associated protein, 91kDa homolog (mouse)
Aliases:		AP180, CALM, DKFZp781O0519, KIAA0656
RefSeq:		NC_000006.11 NT_007299.13
Ensembl:		ENSG00000065609
Entrez:		9892
Chromosome Mapping:		6q14.2

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.998500
y-intercept	35.810000
Efficiency	97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.