PrimePCR™ SYBR® Green Assay: CTD-2135J3.4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR^® Green gene expression analysis.

Info: Same primer pair as used in probe assay qHsaCEP0040281


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PrimePCR™ PreAmp for SYBR® Green Assay: CTD-2135J3.4, Human
Reaction: 400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

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PrimePCR™ Template for SYBR® Green Assay: CTD-2135J3.4, Human
Reaction: 200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.

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Assay Information

Technology:		qPCR
Assay Type:		SYBR® Green
Application:		Gene Expression
Unique Assay ID:		qHsaCED0037558
Assay Design:		exonic
Chromosome Location:		8:10582929-10583071question
Amplicon Length:		113
Splice Variants Targeted:		ENST00000539371 ENST00000304501 ENST00000553390 ENST00000554914 ENST00000264951 ENST00000358077 ENST00000472284 ENST00000408954 ENST00000491893 ENST00000469640 ENST00000430121 ENST00000465829 ENST00000481893 ENST00000306682 ENST00000541662 ENST00000262940 ENST00000461209 ENST00000449970 ENST00000462172 ENST00000522801 ENST00000541884

Gene Information

Description Not Available

Gene Symbol:		CTD-2135J3.4
Gene Name:		SRY (sex determining region Y)-box 7; Uncharacterized protein; cDNA FLJ58508, highly similar to Transcription factor SOX-7
Aliases:		Not Available
RefSeq:		Not Available
Ensembl:		ENSG00000258724
Chromosome Mapping:		Not Available

The below validation information is for the Primer Pair only

Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument	CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent	iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix	SsoAdvanced™ SYBR® Green Supermix
Experimental Sample	qPCR Human Reference Total RNA

Summary Data:

R²	0.999300
y-intercept	36.400000
Efficiency	95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.