PrimePCR™ SYBR® Green Assay: THRB, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028425

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: THRB, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: THRB, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0012393
Assay Design:   Intron-spanning
Chromosome Location:   3:24231728-24338722question
Amplicon Length:   136
Splice Variants Targeted:   ENST00000356447 ENST00000416420 ENST00000415021 ENST00000447875 ENST00000453729 ENST00000431815 ENST00000447414 ENST00000428492 ENST00000418247 ENST00000396671 ENST00000416811

Gene Information

The protein encoded by this gene is a nuclear hormone receptor for triiodothyronine. It is one of the several receptors for thyroid hormone and has been shown to mediate the biological activities of thyroid hormone. Knockout studies in mice suggest that the different receptors while having certain extent of redundancy may mediate different functions of thyroid hormone. Mutations in this gene are known to be a cause of generalized thyroid hormone resistance (GTHR) a syndrome characterized by goiter and high levels of circulating thyroid hormone (T3-T4) with normal or slightly elevated thyroid stimulating hormone (TSH). Several alternatively spliced transcript variants encoding the same protein have been observed for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   THRB
Gene Name:   thyroid hormone receptor, beta (erythroblastic leukemia viral (v-erb-a) oncogene homolog 2, avian)
Aliases:   ERBA-BETA, ERBA2, GRTH, MGC126109, MGC126110, NR1A2, PRTH, THR1, THRB1, THRB2
RefSeq:   NC_000003.11 NT_022517.18 NG_009159.1
Ensembl:   ENSG00000151090
Entrez:   7068
Chromosome Mapping:   3p24.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.610000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download