PrimePCR™ SYBR® Green Assay: CD79A, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0029327

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: CD79A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CD79A, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0014336
Assay Design:   Intron-spanning
Chromosome Location:   19:42383685-42385045question
Amplicon Length:   190
Splice Variants Targeted:   ENST00000221972 ENST00000444740

Gene Information

The B lymphocyte antigen receptor is a multimeric complex that includes the antigen-specific component surface immunoglobulin (Ig). Surface Ig non-covalently associates with two other proteins Ig-alpha and Ig-beta which are necessary for expression and function of the B-cell antigen receptor. This gene encodes the Ig-alpha protein of the B-cell antigen component. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq Jul 2008]

Gene Symbol:   CD79A
Gene Name:   CD79a molecule, immunoglobulin-associated alpha
Aliases:   IGA, MB-1
RefSeq:   NC_000019.9 NG_009619.1 NT_011109.16
Ensembl:   ENSG00000105369
Entrez:   973
Chromosome Mapping:   19q13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.996600
y-intercept 36.890000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download