PrimePCR™ SYBR® Green Assay: TUBA1B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCEP0041407

List Price:    $174.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: TUBA1B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: TUBA1B, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0038685
Assay Design:   exonic
Chromosome Location:   12:49523283-49523379question
Amplicon Length:   67
Splice Variants Targeted:   ENST00000400113 ENST00000336023 ENST00000550367 ENST00000549870 ENST00000547476 ENST00000552984 ENST00000301071 ENST00000295766 ENST00000550767 ENST00000547939 ENST00000546918 ENST00000552924 ENST00000541364 ENST00000301072 ENST00000549183 ENST00000312988 ENST00000321253

Gene Information

Description Not Available

Gene Symbol:   TUBA1B
Gene Name:   tubulin, alpha 1b
Aliases:   K-ALPHA-1
RefSeq:   NC_000012.11 NT_029419.12
Ensembl:   ENSG00000123416
Entrez:   10376
Chromosome Mapping:   12q13.12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 36.040000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download