PrimePCR™ SYBR® Green Assay: ARHGDIB, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0050633

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ARHGDIB, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ARHGDIB, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCED0043371
Assay Design:   exonic
Chromosome Location:   12:15103497-15103590question
Amplicon Length:   64
Splice Variants Targeted:   ENST00000228945 ENST00000536592 ENST00000541644 ENST00000541546 ENST00000545895 ENST00000541380 ENST00000542276

Gene Information

Members of the Rho (or ARH) protein family (see MIM 165390) and other Ras-related small GTP-binding proteins (see MIM 179520) are involved in diverse cellular events including cell signaling proliferation cytoskeletal organization and secretion. The GTP-binding proteins are active only in the GTP-bound state. At least 3 classes of proteins tightly regulate cycling between the GTP-bound and GDP-bound states: GTPase-activating proteins (GAPs) guanine nucleotide-releasing factors (GRFs) and GDP-dissociation inhibitors (GDIs). The GDIs including ARHGDIB decrease the rate of GDP dissociation from Ras-like GTPases (summary by Scherle et al. 1993 [PubMed 8356058]).[supplied by OMIM Dec 2010]

Gene Symbol:   ARHGDIB
Gene Name:   Rho GDP dissociation inhibitor (GDI) beta
Aliases:   D4, GDIA2, GDID4, LYGDI, Ly-GDI, RAP1GN1, RhoGDI2
RefSeq:   NC_000012.11 NT_009714.17
Ensembl:   ENSG00000111348
Entrez:   397
Chromosome Mapping:   12p12.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998200
y-intercept 35.860000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download