PrimePCR™ SYBR® Green Assay: LIFR, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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PrimePCR™ PreAmp for SYBR® Green Assay: LIFR, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: LIFR, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0012563
Assay Design:   Intron-spanning
Chromosome Location:   5:38510737-38511947question
Amplicon Length:   110
Splice Variants Targeted:   ENST00000263409 ENST00000453190

Gene Information

This gene encodes a protein that belongs to the type I cytokine receptor family. This protein combines with a high-affinity converter subunit gp130 to form a receptor complex that mediates the action of the leukemia inhibitory factor a polyfunctional cytokine that is involved in cellular differentiation proliferation and survival in the adult and the embryo. Mutations in this gene cause Schwartz-Jampel syndrome type 2 a disease belonging to the group of the bent-bone dysplasias. A translocation that involves the promoter of this gene t(5;8)(p13;q12) with the pleiomorphic adenoma gene 1 is associated with salivary gland pleiomorphic adenoma a common type of benign epithelial tumor of the salivary gland. Multiple splice variants encoding the same protein have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   LIFR
Gene Name:   leukemia inhibitory factor receptor alpha
Aliases:   CD118, FLJ98106, FLJ99923, LIF-R, SJS2, STWS, SWS
RefSeq:   NC_000005.9 NT_006576.16 NG_011817.1
Ensembl:   ENSG00000113594
Entrez:   3977
Chromosome Mapping:   5p13-p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.660000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download