PrimePCR™ SYBR® Green Assay: ACSL1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0028511

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ACSL1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ACSL1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0012585
Assay Design:   Intron-spanning
Chromosome Location:   4:185678997-185681611question
Amplicon Length:   149
Splice Variants Targeted:   ENST00000454703 ENST00000515030 ENST00000503407 ENST00000281455 ENST00000507295 ENST00000504342 ENST00000513317 ENST00000437665

Gene Information

The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity subcellular localization and tissue distribution all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters and thereby play a key role in lipid biosynthesis and fatty acid degradation. [provided by RefSeq Jul 2008]

Gene Symbol:   ACSL1
Gene Name:   acyl-CoA synthetase long-chain family member 1
Aliases:   ACS1, FACL1, FACL2, LACS, LACS1, LACS2
RefSeq:   NC_000004.11 NT_016354.19
Ensembl:   ENSG00000151726
Entrez:   2180
Chromosome Mapping:   4q35

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.430000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download